ABSTRACT
Objective: To explore the expression charateristics of long non coding RNA bladder cancer associated transcript-1 (BLACAT1) in the cancer tissue and cancer cells in the patients with non small cell lung cancer (NSCLC) and the regulation mechanism, and to elucidate the effect and clinical significance of BLACAT1 in the occurrence and development of NSCLC. Methods: Gene Expression Omnibus (GEO) database was used to analyze the expression characteristics of BLACAT1 in the NSCLC tissue. Kmplot website was used to analyze the correlations between the expression of BLACAT1 and the survival and prognosis of the NSCLC patients. Real time quantitative PCR (qRT PCR) method was applied to detect the expressions of BLACAT1 in cancer tissue and corresponding adjacent normal tissue and NSCLC cell lines of the NSCLC patients. The specific small interfering RNA for BLACAT1 (si BLACAT1 group) or negative control sequence C si NC group) were transfected into the A549 cells. The mRNA expression level of BLACAT1 in A549 cells in various groups were detected by qRT PCR method; the percentages of A549 cells in different cell cycles were detected by flow cytometry; the clone formation abilities of A549 cells in various groups were detected by cell clone formation experiment. The proliferation activities of cells in various groups were detected by CCK8 assay. qRT PCR and Western blotting methods were used to detect the expression level of CyclinDl and CDKN2B mRNA and protein in the A549 cells in various groups. Results; The results of GSE18842 and GSE19804 in GEO datatase. qRT PCR and Kmplot analysis showed that the expression level of BLACAT1 in NSCLC tissue was significantly increased compared with adjacent normal lung tissue C P< 0.05); the survival time in the patients with low expression of BLACAT1 in cancer tissue was significantly longer than that in the patients with high expression of BLACAT1 ( P= 0. 011). Compared with si NC group, the BLACAT1 expression level in the A549 cells in si BLACAT1 group was significantly decreased ( P< 0. 05). Compared with si NC group, the percentage of A459 cells in Gi phase in si BLACAT1 group was increased C P<0. 05) . and the percentage of A549 cells in S phase was decreased ( P<0. 05); the cell clone formation ability and the cell proliferation activity were decreased ( P<-0. 05 or P<0.01). Compared with si NC group, the expression levels of CyclinDl mRNA and protein in the A549 cells in si BLACAT1 group were significantly decreased C P<0. 05). and the expression levels of CDKN2B mRNA and protein were significantly increased C P< 0.05). Conclusion: LncRNA BLACAT1 is up regulated in cancer tissue and cancer cells of the NSCLC patients, and down regulation of BLACAT1 expression can inhibit the proliferation of A549 cells via modulating the CyclinDl/CDKN2B axis, which may serve as a potential therapeutic target for the NSCLC patients.
ABSTRACT
Objective To investigate the protective effect of meglumine adenosine cyclosphosp (MAC) on the cerebral ischemia-reperfusion (I/R) injury in rabbits.Methods Twenty four healthy rabbits were randomly divided into control group (n =6),I/R group (n =6),MAC pretreated group (n =6),and MAC treated group (n =6).Cerebral ischemia-reperfusion injury model was made by separating and electrocoagulating vertebral arteries and clipping common carotid arteries in the latter 3 groups after anesthesia.The sham-operated group underwent vessel separation without clipping.L/R group was administered with nothing,while MAC pretreated group with MAC before ischemia,and MAC treated group with MAC just after ischemia.Blood was gathered from jugular vein before ischemia,and 30 min,1 h,and 2 h after reperfusion for testing IL-8,superoxide dismutase (SOD) and malondialdehyde (MDA).The brain tissue slice was observed by optical microscope.Results Compared to control group and before ischemia,the levels of IL-8 and SOD in serum were significantly increased and decreased,and the levels of MDA was significantly increased at 30 min after reperfusion in I/R group; the levels of IL-8 and MDA in serum were significantly increased,and the levels of SOD in serum was significantly decreased at 1 h and 2 h after reperfusion in I/R group.The levels of IL-8 in serum was less at 30 min and 1 h and 2 h after reperfusion in MAC pretreated group than in I/R group.At 1 h and 2 h after reperfusion,the levels of MDA in serum was less and the levels of SOD in serum was higher in MAC pretreated group than in I/R group.At 1 h and 2 h after reperfusion,the levels of IL-8 in serum were less and the levels of SOD in serum were higher in MAC treated group than in I/R group.The levels of MDA in serum were less at 2 h after reperfusion in MAC treated group than in I/R group.Compared to I/R group,pathological change was lighter in the MAC pretreated and MAC treated group.Conclusions MAC has a fine cerebral-protective effect and has no side effect.
ABSTRACT
AIM:In order to ensure the quality stability of Fructus Polygoni Orientalis,to study the determination method of the fingerprint of Fruetus Polygoni Orientalis and to establish the fingerprint of Fructus Polygoni Orientalis.METHODS:The HPLC assay was used to establish the fingerprint of Fructus Polygoni Orienlalis and 28 pieces of goods were compared.RESULTS:The fingerprint of Fructus Polygoni Orientalis with 7 common peaks was established.The relative retention time and the ranges of relative area of the common peaks were determined.CONCLUSION:The established fingerprint can be used for the quality control of Fructus Polygoni Orientalis.